HPV Virus Scientific Research

Deligdisch L, de Resende Miranda CR, Wu HS, Gil J. Department of Pathology, Mount Sinai Medical Center, New York, NY 10029, USA. liane_deligdisch@mssm.edu

OBJECTIVE: To evaluate the cytopathic effect of human papillomavirus (HPV) infection in adolescents. METHODS: Cervical biopsies from 100 patients, 50 from adolescents age 14 to 20 and 50 from mature women age 35 to 64, all diagnosed with HPV-related lesions (condylomas), were studied histologically and morphometrically. Fifty were associated with low-grade squamous intraepithelial lesions and 50 with high-grade squamous intraepithelial lesions. RESULTS: Epithelial cells with large hyperchromatic, often bizarre-shaped, nuclei, staining positive for HPV-16, were identified in most samples from adolescents. These nuclei were, on average, almost twice as large as those seen in biopsies from older women (P = 0.003), and they mostly occupied the lower half of the epithelium (P = 0.0008). These large cells were further analyzed for texture by a novel image-analysis approach, the autocorrelation factor beta, that revealed a markedly different, smoother nuclear structure, suggestive of a large viral load, different from the irregular chromatin pattern seen in dysplastic nuclei. CONCLUSIONS: These peculiar nuclei were ubiquitous in the adolescent cervical biopsies and probably represent a primary abundant productive viral infection. They should not necessarily be interpreted as dysplastic.

Curr Womens Health Rep. 2002 Dec;2(6):468-75.

Human papillomavirus and adolescent girls.

Rager KM, Kahn JA. Division of Adolescent Medicine, Cincinnati Children's Hospital Medical Center, 3333 Burnett Avenue, Cincinnati, OH 45229, USA.

Human papillomavirus (HPV) infection is one of the most common sexually transmitted infections (STI) in young women. Infection frequently is asymptomatic, but may lead to anogenital condylomata and cervical squamous cell carcinoma. This article provides an overview of the recent literature on HPV infection and its clinical sequelae with a focus on issues pertinent to adolescents.

Publication Types:
• Review
• Review, Tutorial

Eur J Gynaecol Oncol. 2002;23(5):460-2.

Cervical biopsy specimens and human papilloma virus positivity in patients with external genital warts.

Akdeniz S, Yaldiz M, Akdeniz N.

Cervical biopsy specimens were taken from 16 women with external genital warts in order to diagnose concomitant cervical human papillomavirus (HPV) infection and cervical intraepithelial neoplasia (CIN) according to pathology. Positive HPV infection was detected 56% (9/16) of the patients. Among those patients 66% (6/9) had concurrent subclinical low-grade CIN lesions. One of those patients had a high-grade CIN lesion. It is concluded that cervical HPV infection was rather common in women with external genital warts and it is valuable to examine cervical HPV infection by biopsy to detect concomitant CIN.

Ginekol Pol. 2002 Oct;73(10):856-60.

The coexistence of vulvovaginal warts and cervical dysplasia with HPV infection and cervicitis

HPV Virus Scientific Research - Article in Polish

We present a case of 30-year old female patient with persistent cervicitis and vulvovaginal warts. In the course of diagnosis were found cervical dysplasia and a HPV infection type 6 and 11. In therapy we applied antibiotics, chemical therapy of the warts, laser vaporization of the warts, laser conisation, immunotherapy. Three years' intensive therapy didn't lead to satisfactory results. Finally hysterectomy led to the acceptable therapeutical effect.

Publication Types:
• Case Reports

J Huazhong Univ Sci Technolog Med Sci. 2002;22(2):171-3.

Telomerase activity in condyloma acuminatum tissue with different HPV types.

Tu Y, Chen S, Fan C, Lin N, Liu H, Liu Z. Department of Dermatology, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022.

The telomerase activity in condyloma acuminatum (CA) tissue with human papillomavirus (HPV) types of 6/11 and 16/18 was detected to investigate the function of telomerase in the occurrence, development and carcinogenesis of genital CA. Forty-two biopsies from patients with genital CA and 30 control tissue samples were tested for telomerase activity, HPV presence and types. The telomerase activity was determined by modified telomerase repeat amplification protocol (TRAP) assay and HPV typing by polymerase chain reaction (PCR) with typing-specific primers. Results showed that HPV-DNA was negative and the expression rate of telomerase was 16.7% in all normal skin samples. All CA samples were positive for HPV (6/11 type was found in 32 cases, 16/18 in 3 and mixed type in 7). Telomerase activity was detectable in all CA patients. The telomerase activity in CA of 16/18 type was apparently higher than in CA of 6/11 type. It was concluded that the hyperplasia in CA might be increased as a result of HPV infection, suggesting that the activation of telomerase by HPV, especially by 16/18 type may play a role in the etiology and carcinogenesis of genital CA.

J Med Virol. 2003 Mar;69(3):441-6.

IgG antibodies to human papillomavirus 16, 52, 58, and 6 L1 capsids: case-control study of cervical intraepithelial neoplasia in Japan.

Matsumoto K, Yoshikawa H, Yasugi T, Nakagawa S, Kawana K, Takeoka A, Yaegashi N, Iwasaka T, Kanazawa K, Taketani Y, Kanda T. Department of Obstetrics and Gynecology, University of Tokyo, Tokyo, Japan. matsumok@mui.biglobe.ne.jp

In Japan, human papillomavirus (HPV) 16, 52, and 58 are most commonly associated with cervical intraepithelial neoplasia (CIN). By contrast, HPV6 is primarily associated with genital warts. This study was designed to evaluate the association between IgG antibody responses to common HPVs and the risk of CIN development within a Japanese population. CIN cases (n = 141) and controls (n = 109) were tested for cervical HPV DNA and serum IgG antibodies to L1 capsids of HPV16, 52, 58, and 6. Seropositivity to HPV16, 52, and 58 L1 capsids was significantly higher in CIN cases than in controls: 27%, 21%, and 31% versus 16%, 11%, and 11%, respectively (P < 0.05). HPV6 L1 seropositivity was not significantly associated with CIN lesions (P = 0.11). Presence of viral DNA for either HPV16, 52, or 58 correlated with a significant antibody response against the homologous L1 capsids but not heterologous L1 capsids. Furthermore, seropositivity to multiple types of HPV16, 52, and 58 was more strongly associated with an increased risk of CIN development than seropositivity to a single type (P for trend <0.001). These findings indicate that IgG antibodies to L1 capsids of HPV16, 52, and 58 represent an increased risk of CIN development, with antibodies to multiple types being indicative of a further increase in risk. The presence of CIN lesions in women with seropositivity to multiple types suggests that viral exposure to a given type may not be protective against infections by other types and subsequent CIN development. Copyright 2003 Wiley-Liss, Inc.

Akush Ginekol (Sofiia). 2002;41(3):28-31.

Large condylomatous lesions of the vulva and their malignant transformation

HPV Virus Scientific Research - Article in Bulgarian

For the period of 1987 till 2001 were examined 23 women with big condilomatous lesions of the vulva. There were examined the diagnostic and the therapeutical approach and in the last few years the type of HPV in some of these patients. Our aim was to study and reveal the potential for malignant transformation of the big condimatous lesions of the vulva. Different histological types squamous cell cancer of the vulva were found in 18 women (78.26%): condilomatous cancers--9, verucous--7 and basaloid types--2 vulvar cancer. HPV type 16 was found in 6 cases (4 condilomatous and 2 basaloid cancers). HPV type 6 was revealed in 6 cases with verucous cancer, type 11 in 1 case with verucous and 1 case with condilomatous cancer. In 1 case with condilomatous cancer we found HPV type 18. We used mostly radical vulvectomy with bilateral inguinofemoral lymph dissection a modo Ducuing. This kind of operation was performed in all women with the exception of the benign lesions with negative lymph nodes, where simple vulvectomy was mostly used. Wide local excision was used in 1 woman with verucous cancer and in 1 patient with basaloid cancer hemivulvectomy was performed.

Publication Types:
• Case Reports

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